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pyrosequencing-based microbiome analysis  (Pyrosequencing Inc)
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Pyrosequencing Inc pyrosequencing-based microbiome analysis
Microbial diversity and profile in cancer patients (‘diseased’) did not differ significantly from healthy controls, but healthy individuals formed a sub-cluster within the diseased cohort. ( a ) No alterations were observed in alpha diversity between the two groups at the RSV level using ANOVA. ( b ) <t>Microbiome</t> profile of healthy controls clustered within the diseased subjects in a PCoA plot (RSV level). ( c ) LEfSe analysis of taxa associated with healthy subjects and SCC, including the top 100 most abundant genera. ( d – h ) Relative abundance of the genera Bifidobacterium , Pasteurellaceae , Eubacterium , Veillonella, and Fusobacterium in healthy subjects compared to patients.
Pyrosequencing Based Microbiome Analysis, supplied by Pyrosequencing Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Microbial diversity and profile in cancer patients (‘diseased’) did not differ significantly from healthy controls, but healthy individuals formed a sub-cluster within the diseased cohort. ( a ) No alterations were observed in alpha diversity between the two groups at the RSV level using ANOVA. ( b ) Microbiome profile of healthy controls clustered within the diseased subjects in a PCoA plot (RSV level). ( c ) LEfSe analysis of taxa associated with healthy subjects and SCC, including the top 100 most abundant genera. ( d – h ) Relative abundance of the genera Bifidobacterium , Pasteurellaceae , Eubacterium , Veillonella, and Fusobacterium in healthy subjects compared to patients.

Journal: Scientific Reports

Article Title: Preliminary insights into the impact of primary radiochemotherapy on the salivary microbiome in head and neck squamous cell carcinoma

doi: 10.1038/s41598-020-73515-0

Figure Lengend Snippet: Microbial diversity and profile in cancer patients (‘diseased’) did not differ significantly from healthy controls, but healthy individuals formed a sub-cluster within the diseased cohort. ( a ) No alterations were observed in alpha diversity between the two groups at the RSV level using ANOVA. ( b ) Microbiome profile of healthy controls clustered within the diseased subjects in a PCoA plot (RSV level). ( c ) LEfSe analysis of taxa associated with healthy subjects and SCC, including the top 100 most abundant genera. ( d – h ) Relative abundance of the genera Bifidobacterium , Pasteurellaceae , Eubacterium , Veillonella, and Fusobacterium in healthy subjects compared to patients.

Article Snippet: One exception is the study by Hu et al. in 2013 , in which they performed a pyrosequencing-based microbiome analysis of plaque samples taken from subjects undergoing head-and-neck radiotherapy.

Techniques:

Radiochemotherapy in cancer patients substantially impacts the microbial profile. ( a ) Shannon Diversity Index did not reveal significant differences in the salivary microbiome between patients before and after therapy at the RSV level using ANOVA. ( b ) Treatment had a significant impact on the microbiome profile (RDA plot, RSV level). ( c ) Bar plot of the microbiome composition before and after therapy, including the 35 most abundant genera. Taxa are displayed from bottom to top. ( d ) Specific taxa that were significantly increased before or after therapy are plotted in a LEfSe including the 35 most abundant genera.

Journal: Scientific Reports

Article Title: Preliminary insights into the impact of primary radiochemotherapy on the salivary microbiome in head and neck squamous cell carcinoma

doi: 10.1038/s41598-020-73515-0

Figure Lengend Snippet: Radiochemotherapy in cancer patients substantially impacts the microbial profile. ( a ) Shannon Diversity Index did not reveal significant differences in the salivary microbiome between patients before and after therapy at the RSV level using ANOVA. ( b ) Treatment had a significant impact on the microbiome profile (RDA plot, RSV level). ( c ) Bar plot of the microbiome composition before and after therapy, including the 35 most abundant genera. Taxa are displayed from bottom to top. ( d ) Specific taxa that were significantly increased before or after therapy are plotted in a LEfSe including the 35 most abundant genera.

Article Snippet: One exception is the study by Hu et al. in 2013 , in which they performed a pyrosequencing-based microbiome analysis of plaque samples taken from subjects undergoing head-and-neck radiotherapy.

Techniques:

The bacterial and fungal microbiome of healthy subjects and SCC patients is more similar before radiochemotherapy than after. Shannon Diversity of the ( a ) bacterial and ( b ) fungal communities at RSV level (ANOVA). Bar plot of the top 35 most abundant ( c ) bacterial and ( d ) fungal genera. Redundancy analysis of the ( e ) bacterial and ( f ) fungal profiles using ANOVA. Taxa listed in the bar-plot are displayed from bottom to top.

Journal: Scientific Reports

Article Title: Preliminary insights into the impact of primary radiochemotherapy on the salivary microbiome in head and neck squamous cell carcinoma

doi: 10.1038/s41598-020-73515-0

Figure Lengend Snippet: The bacterial and fungal microbiome of healthy subjects and SCC patients is more similar before radiochemotherapy than after. Shannon Diversity of the ( a ) bacterial and ( b ) fungal communities at RSV level (ANOVA). Bar plot of the top 35 most abundant ( c ) bacterial and ( d ) fungal genera. Redundancy analysis of the ( e ) bacterial and ( f ) fungal profiles using ANOVA. Taxa listed in the bar-plot are displayed from bottom to top.

Article Snippet: One exception is the study by Hu et al. in 2013 , in which they performed a pyrosequencing-based microbiome analysis of plaque samples taken from subjects undergoing head-and-neck radiotherapy.

Techniques: